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Ans: (4) Denaturation, Annealing, Extension
Solution: This technique is used for making multiple copies of gene (or DNA) of interest in vitro. Each cycle has three steps:
(2) Primer annealing
(3) Extension of primer
Spooling is (1) Collection of isolated DNA (2) Amplification of DNA (3) Cutting of separated DNA bands from the agarose gel (4) Transfer of separated DNA fragments to synthetic membranes
Select the correct statement from the following : (1) PCR is used for isolation and separation of gene of interest (2) Gel electrophoresis is used for amplification of a DNA segment (3) The polymerase enzyme joins the gene of interest and the vector DNA (4 ...