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The correct order of steps in Polymerase Chain Reaction (PCR) is :

(1) Denaturation, Extension, Annealing

(2) Annealing, Extension, Denaturation

(3) Extension, Denaturation, Annealing

(4) Denaturation, Annealing, Extension

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Ans: (4) Denaturation, Annealing, Extension 

Solution: This technique is used for making multiple copies of gene (or DNA) of interest in vitro. Each cycle has three steps:

(1) Denaturation

(2) Primer annealing

(3) Extension of primer

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