Question

In Recombinant DNA technology antibiotics are used

(1) As selectable markers

(2) To keep medium bacteria-free

(3) to detect alien DNA

(4) To impart disease-resistance to the host plant

Comments

Biotechnology principles and processes

Answer 1

Ans: (2) To keep medium bacteria-free

Sol. The correct answer is option (2) because antibiotics are used in RDT to keep medium or culture bacteria-free.

Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. They undergo insertional inactivation upon addition of alien DNA in their ORF.

Answer 2

Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli