Question

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

(a) The smaller the fragment size, the farther it moves.

(b) Positively charged fragments move to farther end.

(c) Negatively charged fragments do not move.

(d) The larger the fragment size, the farther it moves.

Answer 1

Ans : (a) The smaller the fragment size, the farther it moves.

Explanation :

The DNA fragments separate according to their size through sieving effect provided by the agarose gel.  Hence, the smaller the fragment size the farther it moves.