Question

Spooling is

(1) Collection of isolated DNA

(2) Amplification of DNA

(3) Cutting of separated DNA bands from the agarose gel

(4) Transfer of separated DNA fragments to synthetic membranes

Answer 1

Answer (1) Collection of isolated DNA

Sol. Option (1) is correct answer as spooling refers to wrapping of DNA around a glass rod while extracting DNA. Option (2) is incorrect as amplification is done by PCR. Cutting of separated DNA bands from the agarose gel is elution. Transfer of separated DNA fregments to synthetic memberane is done in blotting.