Question

Select the correct statement from the following :

(1) PCR is used for isolation and separation of gene of interest

(2) Gel electrophoresis is used for amplification of a DNA segment

(3) The polymerase enzyme joins the gene of interest and the vector DNA

(4) Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions

Answer 1

Answer (4) Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions

Sol. Option (4) is the correct answer. PCR is used for amplification of DNA. Gel electrophoresis is used for separation of digested DNA fragments. Ligase enzyme joins the gene of interest and vector DNA.